Description of Research Expertise
Research Interests
Cell and tissue mechanics
Intermediate filaments
Fibrous networks
Phosphoinositide signaling
Key words: actin, PIP2, cytoskeleton, gel, vimentin, fibrin, gelsolin.
Description of Research
Our lab studies the physical properties of fibrous networks and relates these properties to the mechanical responses of cells and tissues. In one project, we produce soft materials, usually hydrogels, to which cell adhesion proteins are linked to study how the stiffness of surfaces alters cell structure, function, and growth. Endothelial cells, fibroblasts, neurons and astrocytes each show unique dependence on both elastic and viscous properties of the substrate, and we seek to understand how they sense and respond to this mechanical cue. In related work we measure the structure and mechanics of cytoskeletal polymers using a variety of imaging, scattering, and rheologic methods. Further studies examine how changes in cell membrane structure mediated by inositol phospholipids lead to production of signals that remodel the cytoskeleton.
Rotation Projects
- Image morphology and cytoskeletal changes in actin or vimentin expressing fibroblasts, endothelial cells, and other cell types grown of soft materials.
- Fluorescence and atomic force microscopy of purified cytoskeletal polymers and associated motor proteins.
- Physical properties of fibrous networks, including those formed by cytoskeletal and extracellular matrix proteins
Lab personnel:
Robert Bucki
Visiting Professor
Medical University of Bialystok
Fitzroy (Jeff) Byfield
Research Specialist
Behnaz Eftekhari
Ph.D. Student, Bioengineering
Daniel Iwamoto
Post-doc
Katherine Kerr
Undergrad, REU and Purdue Univ.
Emile Kraus
Ph.D. student, Physics
J. Yasha Kresh
Visiting Professor
Drexel Univ. College of Medicine
Kalpana Mandal
Post-doc
Serena Omo-Lamai
Ph.D. student, Bioengineering
Dawei Song
Post-doc
Selected Recent Publications
Patteson AE, Vahabikashi A, Goldman RD, Janmey PA. 2020. Mechanical and Non- Mechanical Functions of Filamentous and Non-Filamentous Vimentin. Bioessays: e2000078.
Chaudhuri O, Cooper-White J, Janmey PA, Mooney DJ, Shenoy VB. 2020. Effects of extracellular matrix viscoelasticity on cellular behaviour. Nature 584: 535-46.
Shivers JL, Feng J, van Oosten ASG, Levine H, Janmey PA, MacKintosh FC. 2020. Compression stiffening of fibrous networks with stiff inclusions. Proc Natl Acad Sci U S A 117: 21037-44.
Charrier EE, Pogoda K, Li R, Park CY, Fredberg JJ, Janmey PA. 2020. A novel method to make viscoelastic polyacrylamide gels for cell culture and traction force microscopy. APL Bioeng 4: 036104.
Fatunmbi O, Bradley RP, Kandy SK, Bucki R, Janmey PA, Radhakrishnan R. 2020. A multiscale biophysical model for the recruitment of actin nucleating proteins at the membrane interface. Soft Matter 16: 4941-54.
van Oosten ASG, Chen X, Chin L, Cruz K, Patteson AE, Pogoda K, Shenoy VB, Janmey PA. 2019. Emergence of tissue-like mechanics from fibrous networks confined by close- packed cells. Nature 573: 96-101.
Engstrom TA, Pogoda K, Cruz K, Janmey PA, Schwarz JM. 2019. Compression stiffening in biological tissues: On the possibility of classic elasticity origins. Phys Rev E 99: 052413.